Regulatory

Part:BBa_K2232021:Design

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-18)


PsspB-SpoVAa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part have a s_mutation site NgoMIV which makes the original part BBa_K911008 compatible with RFC[25].

Source

spoVAA and PsspB from B.subtilis Genome.

References

  • This part created by 2017 SZU-China iGEM team basing on the original part BBa_K911008
  • Cabreramartinez R M, Tovarrojo F, Vepachedu V R, et al. Effects of overexpression of nutrient receptors on germination of spores of Bacillus subtilis.[J]. Journal of Bacteriology, 2003, 185(8):2457-64.
  • Paidhungat M, Setlow P. Localization of a germinant receptor protein (GerBA) to the inner membrane of Bacillus subtilis spores.[J]. Journal of Bacteriology, 2001, 183(13):3982-90.
  • Connors M J, Mason J M, Setlow P. Cloning and nucleotide sequencing of genes for three small, acid-soluble proteins from Bacillus subtilis spores.[J]. Journal of Bacteriology, 1986, 166(2):417-25.